The estimation of oxidized glutathione
نویسندگان
چکیده
منابع مشابه
Endoplasmic reticulum : Reduced and oxidized glutathione
6 Division of Molecular and Systems Toxicology, Department of Pharmaceutical 7 Sciences, University of Basel, 4056 Basel, Switzerland 8 2 Swiss Center for Applied Human Toxicology, University of Basel, 4056 Basel, 9 Switzerland 10 3 INRES – Chemical Signalling, University of Bonn, 53113 Bonn, Germany 11 4 Department of Biology, University of Copenhagen, 2200 Copenhagen N, Denmark 12 5 D...
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The reduced glutathione of normal and glucose-6-P dehydrogenase-deficient human erythrocytes was oxidized by subjecting the cells to HzOz diffusion or to treatment with methyl phenylazoformate. A portion of the oxidized glutathione thus formed disappeared from the erythrocytes and could be recovered from the medium when the normal cells were incubated in the absence of glucose or when glucose6-...
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Crude extracts of pea shoots (Pisum sativum) catalyzed oxidized glutathione (GSSG)-dependent oxidation of NADPH which was attributed to NADPH-specific glutathione reductase. The pH optimum was 8 and the K(m) values for GSSG and NADPH were 23 mum and 4.9 mum, respectively. Reduced glutathione (GSH) inhibited the reaction. Crude extracts also catalyzed NADPH-dependent reduction of GSSG; the ratio...
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Oxidized l-glutathione was esterified to the tetra methyl ester using thionyl chloride in methanol solvent. Other alcohols were tested and the reaction progress was monitored via ESI-MS. This procedure proved to be compatible with other small peptides not containing serine and cysteine residues. In contrast to previously reported methods this procedure provided convenient access to esterified p...
متن کاملTransport of Oxidized Glutathione into Barley Vacuoles: Evidence for the Involvement of the Glutathione-S-Conjugate ATPase
Roberto Tommasini, Enrico Martinoia, Erwin Grill, Karl-Josef Dietz*, and Nikolaus Amrhein Institut für Pflanzenwissenschaften, ETH Zürich, Sonneggstraße 5, CH-8092 Zürich, Switzerland * Julius-von-Sachs-Institut für Biowissenschaften, Mittlerer Dallenbergweg 64, D-97082 Würzburg, Bundesrepublik Deutschland Z. Naturforsch. 48c, 867-871 (1993); received August 23, 1993 Glutathione, Transport, Vac...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1953
ISSN: 0306-3283
DOI: 10.1042/bj0550714